Charles University, known also as Charles University in Prague (Univerzita Karlova in Czech), was founded by a charter issued on 7 April 1348 by Charles IV, King of Bohemia and King of the Romans, as the first Studium generale north of the Alps and east of Paris. Charles University is thus one of the oldest European universities.

Czech Technical University in Prague (Czech: České vysoké učení technické v Praze, ČVUT) is one of the largest universities in the Czech Republic, and is one of the oldest institutes of technology in Central Europe. It is also the oldest technical university in Europe.

Czech University of Life Sciences Prague (Česká zemědělská univerzita v Praze) was established in 1906. Currently the university has more than 18 000 students (10% are international students). University has range of study programmes educated in English.

Masaryk University  is the second largest university in the Czech Republic, a member of the Compostela Group and the Utrecht Network. Founded in 1919 in Brno as the third Czech university (after Charles University established in 1348 and Palacký University existent in 1573–1860 It is named after Tomáš Garrigue Masaryk, the first president of an independent Czech Republic. Since 1922, over 171,000 students graduated from the university.

Palacký University Olomouc is a university with long-standing tradition. Founded in the 16th century, it is the oldest university in Moravia and the second-oldest university in the Czech Republic. Today it is a modern higher education facility with a wide range of study programmes and copious scientific and research activities.